| are a number of laboratory tests that a | | | | present with arthritis, a CPK is a good test to order. |
| rheumatologist may order when seeing a patient for | | | | Thyroid function tests. Thyroid disease often is |
| the first time. This is particularly true if rheumatoid | | | | present in patients with inflammatory arthritis. It is |
| arthritis (RA) is suspected. The reason so many | | | | because autoimmunity seems to be involved in the |
| laboratory tests are needed is because of the | | | | development of both of these problems. An |
| complexity involved in arriving at the correct | | | | underactive thyroid (hypothyroidism) can also cause |
| diagnosis. Oftentimes, diseases can look similar. | | | | aches and pains and “muddy the diagnostic |
| Disease may evolve over time and sometimes they | | | | waters”. With severe thyroid disease, |
| overlap. | | | | elevations in CPK may be seen. |
| The following is a rundown of the different tests | | | | Complete Blood Cell Count (CBC). Chronic |
| that can be ordered and why. | | | | inflammation can lead to an anemia. This is a common |
| Erythrocyte Sedimentation Rate (ESR). This test-also | | | | occurrence in patients with rheumatoid arthritis. Also, |
| known as the “sed rate” measures | | | | some of the non-steroidal anti-inflammatory drugs |
| how fast the red blood cells settle to the bottom of | | | | (NSAIDS) which patients with RA take can cause |
| a tube in 1 hour. Inflammation causes the red cells to | | | | ulcers which can lead to blood loss. Also, a low white |
| clump together and therefore settle faster. The rate | | | | blood cell count may be a sign of drug toxicity or |
| in normal individuals is up to 20 mm in 1 hour. | | | | another disease process such as lupus. Also, it is |
| Inflammation increases this rate so that a patient | | | | good to have a baseline result to help with drug |
| with a disorder such as active rheumatoid arthritis will | | | | monitoring |
| often have a sed rate much higher than 20mm. | | | | Chemistry Panel. Viral hepatitis can present with |
| C-Reactive Protein (CRP). CRP measures a protein | | | | inflamed joints. It is important to make sure that liver |
| produced by the liver that is present during acute | | | | function test results are normal. It is a good idea to |
| inflammation or infection. The CRP test can be used | | | | have a baseline in case potentially liver damaging |
| to monitor the effectiveness of treatment as well as | | | | medications such as non-steroidal anti-inflammatory |
| to monitor disease flares. | | | | drugs or methotrexate are used. Another organ |
| Both of these tests correlate with x-ray damage due | | | | system, the kidney can also be affected not only by |
| to RA and long-term disability; persistent elevations | | | | diseases such as lupus or Sjogren’s disease |
| of these blood markers suggest a poor prognosis. | | | | but also is a target for drug toxicity. NSAIDS are the |
| ESR is usually elevated in inflammatory arthritis, but | | | | chief culprits. Also, if a patient’s kidney |
| can be less useful than the CRP, because the ESR | | | | function isn’t normal it will affect the rate of |
| rises more slowly and falls to normal more slowly | | | | elimination of some drugs that are used to treat |
| once inflammation is controlled. The CRP tends to rise | | | | rheumatoid arthritis. Drug toxicity then becomes an |
| faster and go down faster than the sed rate in | | | | issue. |
| response to inflammation. Also, in very early | | | | Viral Hepatitis Panel. As mentioned above, viral |
| inflammatory disease, both the ESR and CRP may | | | | hepatitis can present with an inflammatory form of |
| not be elevated, so normal levels do not rule out the | | | | arthritis. Prior to starting any medicine that can |
| presence of significant disease. These tests are | | | | adversely affect the liver, it is important to have a |
| essential to get at baseline, because there is | | | | baseline in regard to chronic hepatitis B and C |
| evidence they may be useful in predicting disease | | | | infections. Before using anti-TNF medications and |
| severity or response to therapy. | | | | rituximab (Rituxan), the rheumatologist must check |
| Rheumatoid Factor (RF). RF is an immunoglobulin. It is | | | | hepatitis status, especially in regards to hepatitis B |
| in the IgM category of antibodies and is directed | | | | because these drugs can cause aggravation of |
| against another type of antibody type called IgG. It is | | | | hepatitis B. |
| present in about 70% of patients with RA. | | | | Urinalysis. A urine sample is studied for protein, red |
| Unfortunately, an elevated level of RF can be seen in | | | | blood cells, white blood cells, or casts. These |
| about 10% of normal people. Additionally, in roughly | | | | abnormalities may indicate kidney damage due to |
| 20% of patients with RA, the RF is not elevated, | | | | lupus or vasculitis. Use of some medications, because |
| and so a negative test result does not rule out RA | | | | they can injure the kidneys, require initial as well as |
| as a cause of the patient's symptoms. The presence | | | | ongoing screening for urinary abnormalities. |
| of RF correlates with aggressive and erosive disease. | | | | Complement levels. Complement is a serum protein |
| And high levels of RF appear to suggest a worse | | | | that is important in the assessment and monitoring of |
| prognosis. RF can take months to develop, and some | | | | different types of autoimmune disease. Lowered |
| RA patients remain negative for RF throughout the | | | | levels of complement (C3, C4) are indicative of |
| course of their illness. | | | | immune complex formation (where an antibody binds |
| Other autoimmune diseases that can be associated | | | | to an antigen- a foreign protein) and complement |
| with a positive RF include systemic lupus | | | | binding. Lupus patients often show decreased levels |
| erythematosus, Sjogren’s disease, | | | | of total complement, which may be helpful in tracking |
| polymyositis, dermatomyositis, scleroderma, and | | | | disease activity. |
| mixed connective tissue disease. Infections and other | | | | X-rays. X-rays of hands, wrists, feet, and knees are |
| diseases can also be associated with a positive RF. | | | | useful for detecting the presence of erosions. If |
| These include sarcoidosis, tuberculosis,endocarditis, | | | | erosions are seen, especially in early disease, this |
| hepatitis (especially hepatitis C), syphilis, osteomyelitis, | | | | suggests the diagnosis of RA or other erosive |
| infectious mononucleosis, and cirrhosis. A positive RF | | | | diseases, and can indicate a more aggressive disease |
| would not usually be seen in types of arthritis such as | | | | process. |
| gout, osteoarthritis, ankylosing spondylitis, and | | | | Magnetic Resonance Imaging (MRI). MRI is more |
| psoriatic arthritis,. | | | | sensitive than x-rays for detecting inflammation |
| Anticyclic Citrullinated Peptide Antibody (anti-CCP). | | | | within the joint and also for detecting early erosions |
| This is a relatively new blood test thaqt helps to | | | | in RA. |
| confirm a diagnosis of RA. Anti-CCP appears to be | | | | Diagnostic Ultrasound (DUS). Ultrasound is a relatively |
| more specific for RA. Anti-CCP antibody is present in | | | | new technology in the realm of musculoskeletal |
| approximately 30% of RF negative RA (seronegative | | | | diseases. It is an effective, fast, and cost-effective |
| RA). Testing with the combination of anti-CCP | | | | means of detecting early inflammation and damage in |
| antibody and RF may be better for ruling out RA | | | | patients with different types of arthritis. |
| than using either test alone. | | | | Chest X-ray. A chest x-ray (CXR) in patients with |
| High levels of anti-CCP are seen in severe and | | | | early RA will likely be normal. However, because |
| progressive disease. | | | | RA-related lung disease can be present early and |
| Antinuclear Antibody (ANA). The ANA test can help | | | | may be difficult to detect on physical exam, it is |
| detect SLE. However, an elevated ANA is not | | | | reasonable to obtain a baseline CXR to evaluate if |
| specific, and can be seen in disorders other than | | | | signs of lung disease are present. In addition, because |
| lupus, including a significant percentage of patients | | | | some agents, such as methotrexate and anti-TNF |
| with RA. More than 95% of patients with lupus have | | | | drugs, which are used to treat RA can lead to lung |
| a positive ANA test. A more specific test for SLE is | | | | toxicity, getting a baseline CXR before starting |
| the presence of antibodies to DNA (anti-DNA). It is | | | | medication therapy is reasonable. |
| unusual to find antibodies to DNA (anti-DNA) in people | | | | Not all of the above tests will necessarily be ordered |
| who do not have lupus. Levels of anti-DNA vary with | | | | at the first visit. But this is a good basic list that will |
| disease activity. | | | | give the reader a good idea of what to expect. |
| Antibodies to Sm, RNP, Ro (SSA), La (SSB). Lupus | | | | Another issue is the laboratory. If the rheumatologist |
| patients also have other antibodies to different cell | | | | has his or her own specialty laboratory and it is |
| nuclear components. Antibodies to Sm occur only in | | | | state-certified as a reference lab, I would highly |
| patients with lupus while antibodies to RNP occur in | | | | recommend that a patient get their lab tests done at |
| patients with mixed connective tissue disease, and | | | | that office. A rheumatologist will have the best idea |
| antibodies to Ro and La may occur in patients with | | | | as to lab test interpretation and the |
| Sjogren’s disease. | | | | rheumatologist’s lab will be experienced in |
| Creatine phosphokinase (CPK). CPK is a muscle | | | | dealing with arthritis. They will provide the most |
| enzyme that is elevated in a number of types of | | | | accurate and believable results. All too often, large |
| inflammatory conditions, particularly inflammatory | | | | commercial labs are not used to dealing with the |
| muscle diseases such as polymyositis and | | | | complexity of arthritis testing. A correct diagnosis is |
| dermatomyosotis. Since these conditions can also | | | | key! |